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Micu1 (NM_144822) Mouse Tagged ORF Clone
CAT＃: MG207652
- TrueORF®
Micu1 (tGFP-tagged) - Mouse calcium binding atopy-related autoantigen 1 (Cbara1)
ORF Plasmid: DDK
"NM_144822" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C730016L05Rik; Calc; Cbara1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207652 representing NM_144822
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGTCTTAACACCCTTTCTGCGTTGGCAGAACTAGCTGTGGGCTCTCGCTGGTACCATGGGGCAT CACAGCCCACACAGACCAAACGAAGATTGATGCTGGTGGCGTTCCTGGGAGCATCTGCAGTGACTGCAAG TACCGGTCTCCTGTGGAAGAAGGCTCACGCAGAATCTCCACCATGCGTTAACAGCAAGAAGCCTGACACA GAAGATAAAGAGAGAAACAAAGACAGCGGGGAAGTGTCCAGCCGTGAAGGAAGGGCTGCAGATGCTGCGG CAGAGCCTTATCCTGAGGACAAGAAGAAGAAGCGCTCTGGATTCAGAGACAGAAAAGTGATGGAGTATGA GAATCGAATCCGAGCCTACTCCACACCAGACAAAATTTTCCGATATTTTGCCACCTTGAAAGTAATCAAC GAACCTGGTGAAACCGAAGTGTTCATGACTCCGCAGGACTTTGTGCGCTCCATAACGCCCAATGAGAAGC AGCCAGAACACTTGGGCCTGGATCAGTATATAATAAAGCGCTTTGATGGAAAGAAAATTGCCCAGGAACG AGAAAAATTTGCTGATGAAGGCAGCATCTTCTACAGCCTTGGAGAGTGTGGGCTCATCTCCTTCTCTGAC TACATCTTCCTCACAACAGTCCTCTCCACTCCTCAGAGAAATTTTGAAATTGCTTTCAAGATGTTTGACT TGAATGGAGACGGAGAGGTAGACATGGAGGAGTTTGAGCAGGTTCAGAGCATCATTCGCTCCCAGACCAG CATGGGCATGCGTCACAGAGACCGTCCAACCACTGGGAACACCCTCAAGTCTGGCTTATGTTCGGCCCTC ACGACCTACTTTTTTGGAGCTGATCTCAAAGGGAAACTGACCATCAAAAACTTCCTGGAATTTCAGCGCA AACTTCAGCATGACGTTCTAAAGCTGGAGTTTGAACGCCATGACCCGGTAGATGGGAGAATCTCTGAGAG GCAGTTCGGGGGCATGCTGCTGGCCTACAGTGGAGTGCAGTCCAAGAAGCTGACCGCCATGCAGAGACAG CTGAAGAAGCACTTCAAAGATGGGAAGGGCCTGACTTTCCAGGAGGTAGAGAACTTCTTCACTTTCCTGA AGAACATTAATGACGTGGACACTGCACTAAGCTTTTACCACATGGCTGGAGCATCTCTTGATAAAGTGAC CATGCAGCAAGTGGCCAGGACAGTGGCGAAGGTCGAACTCTCGGACCATGTGTGTGACGTGGTGTTCGCG CTCTTTGACTGTGATGGCAATGGGGAGCTGAGCAACAAGGAGTTCGTTTCCATCATGAAGCAGCGGCTGA TGAGAGGCCTGGAGAAGCCCAAGGACATGGGCTTCACCCGCCTCATGCAGGCCATGTGGAAATGTGCCCA AGAAACTGCCTGGGACTTTGCTCTGCCCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207652 representing NM_144822
Red=Cloning site Green=Tags(s) MFRLNTLSALAELAVGSRWYHGASQPTQTKRRLMLVAFLGASAVTASTGLLWKKAHAESPPCVNSKKPDT EDKERNKDSGEVSSREGRAADAAAEPYPEDKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVIN EPGETEVFMTPQDFVRSITPNEKQPEHLGLDQYIIKRFDGKKIAQEREKFADEGSIFYSLGECGLISFSD YIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSAL TTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRISERQFGGMLLAYSGVQSKKLTAMQRQ LKKHFKDGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFA LFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144822 |
| ORF Size | 1431 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144822.3 |
| RefSeq Size | 2275 bp |
| RefSeq ORF | 1434 bp |
| Locus ID | 216001 |
| UniProt ID | Q8VCX5 |
| Gene Summary | Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains (PubMed:24560927). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake (By similarity). Acts as a gatekeeper of MCU at low concentration of calcium, preventing channel opening (By similarity). Enhances MCU opening at high calcium concentration, allowing a rapid response of mitochondria to calcium signals generated in the cytoplasm (PubMed:24560927). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (By similarity). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201052 | Micu1 (untagged) - Mouse calcium binding atopy-related autoantigen 1 (Cbara1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MR207652 | Micu1 (Myc-DDK-tagged) - Mouse calcium binding atopy-related autoantigen 1 (Cbara1), nuclear gene encoding mitochondrial protein |
CNY 3,330.00 |
|
| MR207652L3 | Lenti ORF clone of Micu1 (Myc-DDK-tagged) - Mouse calcium binding atopy-related autoantigen 1 (Cbara1), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
|
| MR207652L4 | Lenti ORF clone of Micu1 (mGFP-tagged) - Mouse calcium binding atopy-related autoantigen 1 (Cbara1), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
