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Des (NM_010043) Mouse Tagged ORF Clone
CAT＃: MG207502
- TrueORF®
Des (tGFP-tagged) - Mouse desmin (Des)
ORF Plasmid: DDK
"NM_010043" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207502 representing NM_010043
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAGGCCTACTCGTCCAGCCAGCGCGTGTCCTCCTACCGCCGCACCTTCGGCGGCGCCCCGGGCT TCTCTCTGGGCTCCCCGCTGAGCTCTCCCGTGTTCCCTCGAGCAGGCTTCGGTACCAAGGGCTCCTCGAG TTCAATGACATCCCGCGTGTACCAGGTGTCGCGCACGTCGGGCGGGGCTGGAGGCTTGGGGTCGCTGCGG TCTAGCCGGCTGGGGACCACCCGAGCGCCATCCTATGGCGCGGGCGAGCTGCTGGACTTCTCCCTGGCCG ACGCTGTGAACCAGGAGTTCCTGGCCACGCGCACCAACGAGAAGGTGGAGCTGCAAGAGCTCAATGACCG CTTCGCCAACTACATCGAGAAGGTGCGCTTCTTGGAGCAGCAGAACGCCGCGCTCGCCGCCGAGGTCAAC CGGCTCAAGGGCCGCGAACCGACTCGGGTCGCCGAGCTCTACGAGGAGGAGATGCGCGAGCTGCGGCGCC AGGTGGAGGTGCTCACCAACCAGCGCGCCCGGGTCGACGTGGAGCGTGACAACCTGATAGACGACCTGCA GAGGCTCAAGGCCAAACTACAGGAGGAAATCCAACTAAGAGAAGAAGCAGAGAACAACTTGGCTGCCTTC CGAGCGGATGTGGATGCAGCCACTCTAGCTCGTATTGACCTGGAGCGCAGAATCGAATCCCTCAACGAGG AGATCGCGTTCCTTAAGAAAGTGCATGAAGAGGAGATCCGTGAGCTTCAGGCCCAGCTTCAGGAACAGCA GGTCCAGGTGGAGATGGACATGTCCAAGCCGGACCTCACAGCTGCCCTCAGGGACATCCGGGCTCAGTAT GAGACCATCGCGGCTAAGAACATCTCTGAGGCTGAAGAATGGTACAAGTCCAAGGTTTCAGACTTGACTC AGGCAGCCAATAAGAACAACGATGCGCTGCGCCAAGCCAAGCAGGAGATGATGGAATACCGACACCAGAT CCAGTCCTACACCTGCGAGATTGATGCCCTCAAGGGCACCAACGACTCCCTGATGAGGCAGATGAGGGAG CTGGAGGATCGCTTTGCCAGCGAGGCCAATGGCTATCAGGACAACATTGCGCGCCTGGAGGAGGAGATCC GACACCTAAAGGATGAGATGGCCCGCCATCTGCGCGAGTACCAGGACCTGCTCAATGTGAAGATGGCCTT GGATGTGGAGATCGCCACCTACCGGAAGCTACTGGAGGGCGAGGAGAGCAGGATCAACCTTCCTATCCAG ACCTTCTCTGCTCTCAACTTCCGAGAAACCAGCCCCGAGCAAAGGGGTTCTGAAGTCCATACCAAAAAGA CAGTGATGATCAAGACCATTGAGACCCGGGATGGAGAGGTTGTCAGCGAGGCTACACAGCAACAACATGA AGTGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207502 representing NM_010043
Red=Cloning site Green=Tags(s) MSQAYSSSQRVSSYRRTFGGAPGFSLGSPLSSPVFPRAGFGTKGSSSSMTSRVYQVSRTSGGAGGLGSLR SSRLGTTRAPSYGAGELLDFSLADAVNQEFLATRTNEKVELQELNDRFANYIEKVRFLEQQNAALAAEVN RLKGREPTRVAELYEEEMRELRRQVEVLTNQRARVDVERDNLIDDLQRLKAKLQEEIQLREEAENNLAAF RADVDAATLARIDLERRIESLNEEIAFLKKVHEEEIRELQAQLQEQQVQVEMDMSKPDLTAALRDIRAQY ETIAAKNISEAEEWYKSKVSDLTQAANKNNDALRQAKQEMMEYRHQIQSYTCEIDALKGTNDSLMRQMRE LEDRFASEANGYQDNIARLEEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLPIQ TFSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010043 |
| ORF Size | 1407 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010043.2, NP_034173.1 |
| RefSeq Size | 2162 bp |
| RefSeq ORF | 1410 bp |
| Locus ID | 13346 |
| UniProt ID | P31001 |
| Gene Summary | This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane and are essential for maintaining the strength and integrity of skeletal, cardiac and smooth muscle fibers. Mutations in this gene affect assembly of intermediate filaments. Mice lacking this gene are able to develop and reproduce but exhibit abnormal muscle fibers. Mutations in the human gene are associated with myofibrillar myopathy, dilated cardiomyopathy, neurogenic scapuloperoneal syndrome and autosomal recessive limb-girdle muscular dystrophy, type 2R. [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201460 | Des (untagged) - Mouse desmin (Des), (10ug) |
CNY 2,000.00 |
|
| MR207502 | Des (Myc-DDK-tagged) - Mouse desmin (Des) |
CNY 3,330.00 |
|
| MR207502L3 | Lenti ORF clone of Des (Myc-DDK-tagged) - Mouse desmin (Des) |
CNY 5,230.00 |
|
| MR207502L4 | Lenti ORF clone of Des (mGFP-tagged) - Mouse desmin (Des) |
CNY 5,230.00 |
