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Ido1 (NM_008324) Mouse Tagged ORF Clone
CAT＃: MG206394
- TrueORF®
Ido1 (tGFP-tagged) - Mouse indoleamine-pyrrole 2,3 dioxygenase (Indo)
ORF Plasmid: DDK
"NM_008324" in other vectors (6)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ido; Indo |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206394 representing NM_008324
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTCAGTAAAATATCTCCTACAGAAGGTTCTAGAAGGATCCTTGAAGACCACCACATAGATGAAG ATGTGGGCTTTGCTCTACCACATCCACTGGTGGAGCTGCCCGACGCATACAGCCCCTGGGTCCTTGTGGC TAGAAATCTGCCTGTGCTGATTGAGAACGGGCAGCTTCGAGAAGAAGTTGAAAAGCTGCCCACACTGAGC ACGGACGGACTGAGAGGACACAGGTTACAGCGCCTGGCACACCTGGCCCTGGGGTACATCACCATGGCGT ATGTGTGGAACCGAGGGGATGACGATGTTCGAAAGGTGCTGCCCCGCAATATTGCTGTTCCCTACTGCGA GCTCTCAGAGAAGTTGGGCCTGCCTCCTATTCTGTCTTATGCAGACTGTGTCCTGGCAAACTGGAAGAAA AAGGACCCCAATGGGCCCATGACATACGAGAACATGGACATTCTGTTCTCATTTCCTGGTGGGGACTGCG ACAAGGGCTTCTTCCTCGTCTCTCTATTGGTGGAAATCGCAGCTTCTCCTGCAATCAAAGCAATCCCCAC TGTATCCAGTGCAGTAGAGCGTCAAGACCTGAAAGCATTGGAAAAGGCACTGCACGACATAGCTACCAGT CTGGAGAAAGCCAAGGAAATTTTTAAGAGGATGCGTGACTTTGTGGACCCAGACACGTTTTTCCACGTTC TCCGCATATATCTGTCTGGCTGGAAATGCAGCTCCAAGCTGCCAGAAGGTCTGCTGTATGAGGGGGTCTG GGACACCCCAAAAATGTTTTCAGGGGGCAGTGCAGGCCAGAGCAGCATCTTCCAGAGTCTTGATGTCCTT CTGGGAATAAAACACGAGGCTGGCAAAGAATCTCCTGCAGAATTCCTCCAGGAAATGAGAGAGTACATGC CTCCAGCCCACCGGAACTTCCTTTTCTTCTTAGAGTCAGCTCCCCCAGTCCGTGAGTTTGTCATTTCAAG ACACAATGAAGACTTGACGAAAGCTTATAACGAGTGTGTGAATGGTCTGGTCTCTGTGAGAAAGTTCCAC CTCGCAATAGTAGATACTTACATTATGAAACCTTCGAAGAAGAAGCCCACTGATGGCGACAAGTCGGAAG AGCCCTCAAATGTGGAAAGCAGAGGGACTGGGGGTACGAATCCCATGACTTTCCTAAGGAGTGTGAAAGA TACAACCGAGAAAGCTCTTCTGAGTTGGCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206394 representing NM_008324
Red=Cloning site Green=Tags(s) MALSKISPTEGSRRILEDHHIDEDVGFALPHPLVELPDAYSPWVLVARNLPVLIENGQLREEVEKLPTLS TDGLRGHRLQRLAHLALGYITMAYVWNRGDDDVRKVLPRNIAVPYCELSEKLGLPPILSYADCVLANWKK KDPNGPMTYENMDILFSFPGGDCDKGFFLVSLLVEIAASPAIKAIPTVSSAVERQDLKALEKALHDIATS LEKAKEIFKRMRDFVDPDTFFHVLRIYLSGWKCSSKLPEGLLYEGVWDTPKMFSGGSAGQSSIFQSLDVL LGIKHEAGKESPAEFLQEMREYMPPAHRNFLFFLESAPPVREFVISRHNEDLTKAYNECVNGLVSVRKFH LAIVDTYIMKPSKKKPTDGDKSEEPSNVESRGTGGTNPMTFLRSVKDTTEKALLSWP TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008324 |
| ORF Size | 1221 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008324.2 |
| RefSeq Size | 1555 bp |
| RefSeq ORF | 1224 bp |
| Locus ID | 15930 |
| UniProt ID | P28776 |
| Gene Summary | Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity (PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection (Ref. 3).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202318 | Ido1 (untagged) - Mouse indoleamine 2,3-dioxygenase 1 (Ido1), (10ug) |
CNY 2,000.00 |
|
| MR206394 | Ido1 (Myc-DDK-tagged) - Mouse indoleamine 2,3-dioxygenase 1 (Ido1) |
CNY 5,488.00 |
|
| MR206394L1 | Lenti ORF clone of Ido1 (Myc-DDK-tagged) - Mouse indoleamine 2,3-dioxygenase 1 (Ido1) |
CNY 4,750.00 |
|
| MR206394L2 | Lenti ORF clone of Ido1 (mGFP-tagged) - Mouse indoleamine 2,3-dioxygenase 1 (Ido1) |
CNY 7,888.00 |
|
| MR206394L3 | Lenti ORF clone of Ido1 (Myc-DDK-tagged) - Mouse indoleamine 2,3-dioxygenase 1 (Ido1) |
CNY 7,888.00 |
|
| MR206394L4 | Lenti ORF clone of Ido1 (mGFP-tagged) - Mouse indoleamine 2,3-dioxygenase 1 (Ido1) |
CNY 7,888.00 |
