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Tsg101 (NM_021884) Mouse Tagged ORF Clone
CAT＃: MG206104
- TrueORF®
Tsg101 (tGFP-tagged) - Mouse tumor susceptibility gene 101 (Tsg101)
ORF Plasmid: DDK
"NM_021884" in other vectors (4)
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CNY 5,256.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI255943; CC2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206104 representing NM_021884
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGTCCGAGAGTCAGCTGAAGAAGATGATGTCCAAGTACAAATATAGAGATCTAACCGTCCGTC AAACTGTCAATGTCATCGCTATGTACAAAGATCTCAAACCTGTATTGGATTCATATGTTTTTAATGATGG CAGTTCCAGGGAGCTGGTGAACCTCACTGGTACAATCCCAGTGCGTTATCGAGGTAATATATATAATATT CCAATATGCCTGTGGCTGCTGGACACATACCCATATAACCCCCCTATCTGTTTTGTTAAGCCTACTAGTT CAATGACTATTAAAACAGGAAAGCATGTGGATGCAAATGGGAAAATCTACCTACCTTATCTACATGACTG GAAACATCCACGGTCAGAGTTGCTGGAGCTTATTCAAATCATGATTGTGATATTTGGAGAGGAGCCTCCA GTGTTCTCCCGGCCTACTGTTTCTGCATCCTACCCACCATACACAGCAACAGGGCCACCAAATACCTCCT ACATGCCAGGCATGCCAAGTGGAATCTCTGCATATCCATCTGGATACCCTCCCAACCCCAGTGGTTATCC TGGCTGTCCTTACCCACCTGCTGGCCCATACCCTGCCACAACAAGCTCACAGTACCCTTCCCAGCCTCCT GTGACCACTGTTGGTCCCAGCAGAGATGGCACAATCAGTGAGGACACTATCCGTGCATCTCTCATCTCAG CAGTCAGTGACAAACTGAGATGGCGGATGAAGGAGGAAATGGATGGTGCCCAGGCAGAGCTTAATGCCTT GAAACGAACAGAGGAAGATCTGAAAAAAGGCCACCAGAAACTGGAAGAGATGGTCACCCGCTTAGATCAA GAAGTAGCTGAAGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTAAGTTCTGCTCTGG AGAAAATGGAAAATCAATCTGAAAATAATGATATTGATGAAGTTATCATTCCCACAGCCCCACTGTATAA ACAGATTCTAAATCTGTATGCAGAGGAAAATGCTATTGAAGACACTATCTTTTACCTTGGAGAAGCTTTG CGGCGGGGAGTCATAGACCTGGATGTGTTCCTGAAACACGTCCGCCTCCTGTCCCGTAAACAGTTCCAGC TAAGGGCACTAATGCAAAAGGCAAGGAAGACTGCGGGCCTTAGTGACCTCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206104 representing NM_021884
Red=Cloning site Green=Tags(s) MAVSESQLKKMMSKYKYRDLTVRQTVNVIAMYKDLKPVLDSYVFNDGSSRELVNLTGTIPVRYRGNIYNI PICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHDWKHPRSELLELIQIMIVIFGEEPP VFSRPTVSASYPPYTATGPPNTSYMPGMPSGISAYPSGYPPNPSGYPGCPYPPAGPYPATTSSQYPSQPP VTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDGAQAELNALKRTEEDLKKGHQKLEEMVTRLDQ EVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEAL RRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021884 |
| ORF Size | 1173 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021884.4 |
| RefSeq Size | 1833 bp |
| RefSeq ORF | 1176 bp |
| Locus ID | 22088 |
| UniProt ID | Q61187 |
| Gene Summary | Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200893 | Tsg101 (untagged) - Mouse tumor susceptibility gene 101 (Tsg101), (10ug) |
CNY 2,000.00 |
|
| MR206104 | Tsg101 (Myc-DDK-tagged) - Mouse tumor susceptibility gene 101 (Tsg101) |
CNY 3,656.00 |
|
| MR206104L3 | Lenti ORF clone of Tsg101 (Myc-DDK-tagged) - Mouse tumor susceptibility gene 101 (Tsg101) |
CNY 4,750.00 |
|
| MR206104L4 | Lenti ORF clone of Tsg101 (mGFP-tagged) - Mouse tumor susceptibility gene 101 (Tsg101) |
CNY 4,750.00 |
