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Lias (NM_024471) Mouse Tagged ORF Clone
CAT＃: MG205779
- TrueORF®
Lias (tGFP-tagged) - Mouse lipoic acid synthetase (Lias)
ORF Plasmid: DDK
"NM_024471" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 7a5ex; 2900022L22Rik; 4933425M12Rik; C77512; lip-syn; LS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205779 representing NM_024471
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTACGCTGCTGGGATACAGCTCGCAGCCTGGGGTCCCGGATATTTGGAAGATATGCGTTCACGG TTAGAGCATTAAGTTCTTTGCCAGATAAGAAAAAGGAATTTTTGCATAATGGACCAGACCTTCAAGATTT TGTATCTGGTGATCTTGCAGACAAGAGTACATGGGATGAATATAAAGGAAACTTAAAGCGCCAGAAAGGA GAAAGGCTAAGACTACCTCCATGGCTAAAGACAAAGATACCCATGGGTAAAAACTACAATAAACTGAAAA ATACATTGCGGAATTTAAGTCTCCACACAGTGTGTGAGGAAGCCCGGTGCCCCAACATTGGAGAGTGTTG GGGAGGTGGGGAATATGCCACAGCCACAGCCACGATCATGTTGATGGGGGACACATGCACAAGAGGTTGC AGATTTTGTTCCGTTAAGACCGCAAGAAATCCCCCTCCATTGGATGCCAATGAGCCCGACAATACGGCCA AAGCAATTGCAGAGTGGGGTCTGGATTATGTTGTCCTGACGTCGGTGGATCGAGATGATGTGGCCGACGG GGGAGCTGAGCACATCGCCAAGACCGTGTCATGCTTGAAGGAAAGGAATCCAAAAATCCTCGTGGAATGC CTCACCCCAGACTTCCGAGGTGATCTGAGAGCAGTGGAGAAGGTGGCTCTGTCTGGATTAGATGTGTATG CACACAATGTGGAGACTGTCCCCGAGTTACAGAGGAAAGTTCGTGATCCCCGGGCCAATTTTGACCAGTC TCTTCGTGTACTGAGACATGCCAAGGAGGTCCAGCCTGATGTTGTTTCCAAAACATCGATAATGCTGGGC CTTGGTGAGACAGACGAGCAAGTCTATGCCACACTGAAAGCTCTCCGTGCGGCCGACGTGGACTGTTTAA CTCTAGGGCAGTACATGCAGCCAACGAAGCGCCACCTTAAGGTTGAAGAATATGTTACTCCTGAGAAGTT CAAATACTGGGAAAAAGTAGGGAATGAACTTGGATTTCTCTATACGGCAAGTGGTCCTTTGGTGCGATCT TCATATAAAGCAGGTGAATTTTTCCTGAAAAATCTAGTGGCTAGAAGAAAAACAAAAGCTTCTAAAGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205779 representing NM_024471
Red=Cloning site Green=Tags(s) MALRCWDTARSLGSRIFGRYAFTVRALSSLPDKKKEFLHNGPDLQDFVSGDLADKSTWDEYKGNLKRQKG ERLRLPPWLKTKIPMGKNYNKLKNTLRNLSLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGC RFCSVKTARNPPPLDANEPDNTAKAIAEWGLDYVVLTSVDRDDVADGGAEHIAKTVSCLKERNPKILVEC LTPDFRGDLRAVEKVALSGLDVYAHNVETVPELQRKVRDPRANFDQSLRVLRHAKEVQPDVVSKTSIMLG LGETDEQVYATLKALRAADVDCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEKVGNELGFLYTASGPLVRS SYKAGEFFLKNLVARRKTKASKV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024471 |
| ORF Size | 1665 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024471.5, NP_077791.1 |
| RefSeq Size | 1669 bp |
| RefSeq ORF | 1122 bp |
| Locus ID | 79464 |
| UniProt ID | Q99M04 |
| Gene Summary | Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207779 | Lias (untagged) - Mouse lipoic acid synthetase (Lias), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
| MR205779 | Lias (Myc-DDK-tagged) - Mouse lipoic acid synthetase (Lias), nuclear gene encoding mitochondrial protein |
CNY 2,850.00 |
|
| MR205779L3 | Lenti ORF clone of Lias (Myc-DDK-tagged) - Mouse lipoic acid synthetase (Lias), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR205779L4 | Lenti ORF clone of Lias (mGFP-tagged) - Mouse lipoic acid synthetase (Lias), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
