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Tmem16a (BC006062) Mouse Tagged ORF Clone
CAT＃: MG205066
- TrueORF®
Tmem16a (tGFP-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765)
ORF Plasmid: DDK
"BC006062" in other vectors (4)
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CNY 2,090.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Tmem16a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205066 representing BC006062
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGTGTGCCCCGGGCGGCTGCCTCATGGAGCTCTGTATCCAGCTGAGCATCATTATGCTGGGCA AGCAGCTAATCCAGAACAATCTCTTCGAGATTGGCATCCCGAAGATGAAAAAGTTCATCCGCTACCTGAA GCTGCGCAGACAGAGCCCCTCAGACCGTGAAGAGTACGTGAAGCGGAAGCAGCGCTATGAGGTGGACTTC AACCTCGAACCTTTCGCCGGCCTCACGCCCGAGTACATGGAAATGATCATTCAGTTCGGCTTTGTCACCC TGTTTGTTGCGTCCTTCCCTCTGGCTCCACTCTTCGCCCTGCTAAACAACATCATTGAGATCCGCCTGGA TGCCAAAAAGTTTGTCACCGAGCTACGGAGGCCAGTAGCCATCAGAGCCAAAGACATCGGCATCTGGTAT AACATCCTCAGAGGTGTTGGGAAGCTGGCTGTCATCATTAATGCCTTTGTGATCTCCTTCACGTCTGACT TCATCCCTCGCCTGGTGTACCTCTACATGTACAGTCAGAATGGGACCATGCACGGCTTCGTCAATCACAC GCTCTCTTCCTTCAATGTCAGCGACTTCCAGAATGGCACAGCACCCAATGACCCACTGGACCTGGGCTAT GAGGTTCAGATCTGCAGGTATAAAGATTACCGGGAACCCCCATGGTCAGAACACAAGTATGACATCTCCA AAGACTTCTGGGCTGTCCTGGCCGCCCGACTGGCATTTGTCATTGTCTTCCAGAACCTGGTGATGTTCAT GAGTGACTTTGTGGACTGGGTGATCCCTGATATCCCCAAAGACATCAGCCAGCAGATCCACAAAGAGAAG GTTCTCATGGTGGAGCTGTTTATGCGTGAGGAGCAGGGCAAGCAGCAGCTACTGGACACATGGATGGAGA AGGAGAAGCCAAGGGATGTGCCTTGTAACAACCACAGCCCCACAACCCACCCAGAGGCAGGCGACGGCAG CCCAGTCCCCAGCTACGAGTACCATGGGGACGCACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205066 representing BC006062
Red=Cloning site Green=Tags(s) MEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDF NLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWY NILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGY EVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEK VLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC006062 |
| ORF Size | 1019 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC006062, AAH06062 |
| RefSeq Size | 2473 bp |
| RefSeq ORF | 1019 bp |
| Locus ID | 101772 |
| Gene Summary | Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Binding of plasma membrane lipids recruits the yeast integral membrane protein Ist2 to the cortical ER.
,null,
Traffic (Copenhagen, Denmark)
,PubMed ID 19453974
[Tmem16a]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200539 | Tmem16a (untagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765), (10ug) |
CNY 2,000.00 |
|
| MR205066 | Tmem16a (Myc-DDK-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) |
CNY 1,900.00 |
|
| MR205066L3 | Lenti ORF clone of Tmem16a (Myc-DDK-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) |
CNY 3,800.00 |
|
| MR205066L4 | Lenti ORF clone of Tmem16a (mGFP-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) |
CNY 3,800.00 |
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