Icam2 (NM_010494) Mouse Tagged ORF Clone

CAT#: MG203730

  • TrueORF®

Icam2 (tGFP-tagged) - Mouse intercellular adhesion molecule 2 (Icam2)

ORF Plasmid: DDK tGFP



  "NM_010494" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


CD102 Rabbit pAb
    • 100 ul

CNY 2,640.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms CD102; Icam-2; Ly-60
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG203730 representing NM_010494
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTCTTTTGCTTGCTGGAGCCTGTCTCTTCTTATCCTGTTCTACAGCCCAGGGTCTGGTGAGAAGG
CCTTTGAGGTCTACATATGGTCCGAGAAGCAGATAGTAGAAGCCACAGAGTCTTGGAAAATCAACTGCAG
CACCAACTGCGCAGCCCCAGACATGGGCGGCCTGGAGACGCCCACGAATAAAATAATGTTGGAAGAGCAT
CCTCAAGGGAAGTGGAAACAGTTCTTAGTCTCAAACGTCTCCAAAGACACGGTCTTCTTTTGCCATTTCA
CGTGTTCGGGAAAGCAGCACTCGGAGAGTCTCAACATCAGGGTGTACCAGCCTCCAGCTCAAGTCACACT
GAAGCTGCAGCCGCCTCGGGTGTTTGTGGGTGAAGACTTCACCATTGAGTGCACGGTGTCCCCTGTGCAG
CCCCTTGAGAGGCTCACCCTCTCTCTGCTCCGTGGCAGAGAGACCCTGAAGAATCAGACCTTTGGGGGAG
CAGAAACTGTCCCCCAAGAGGCCACAGCCACGTTCAACAGCACAGCTCTGAAAAAGGACGGTCTCAACTT
TTCCTGCCAGGCTGAGCTGGATCTACGGCCCCATGGTGGGTATATCATCCGCAGCATCTCGGAGTACCAG
ATCCTTGAAGTCTATGAGCCGATGCAGGACAACCAAATGGTCATCATCATCGTGGTGGTGTCAATACTGC
TGTTCTTATTTGTGACATCTGTCCTGCTATGCTTTATCTTTGGCCAGCACTGGCACAGAAGACGGACAGG
CACCTACGGGGTGCTAGCTGCCTGGAGGAGGCTGCCCCGAGCCTTTCGGGCACGTCCCGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG203730 representing NM_010494
Red=Cloning site Green=Tags(s)

MSSFACWSLSLLILFYSPGSGEKAFEVYIWSEKQIVEATESWKINCSTNCAAPDMGGLETPTNKIMLEEH
PQGKWKQFLVSNVSKDTVFFCHFTCSGKQHSESLNIRVYQPPAQVTLKLQPPRVFVGEDFTIECTVSPVQ
PLERLTLSLLRGRETLKNQTFGGAETVPQEATATFNSTALKKDGLNFSCQAELDLRPHGGYIIRSISEYQ
ILEVYEPMQDNQMVIIIVVVSILLFLFVTSVLLCFIFGQHWHRRRTGTYGVLAAWRRLPRAFRARPV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010494
ORF Size 831 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010494.2
RefSeq Size 1168 bp
RefSeq ORF 834 bp
Locus ID 15896
UniProt ID P35330
Gene Summary ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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