Psma7 (NM_011969) Mouse Tagged ORF Clone

CAT#: MG203141

  • TrueORF®

Psma7 (tGFP-tagged) - Mouse proteasome (prosome, macropain) subunit, alpha type 7 (Psma7)

ORF Plasmid: DDK tGFP



  "NM_011969" in other vectors (6)

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Mouse Protein / Lysate Request

CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms C6-I
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG203141 representing NM_011969
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTACGACCGCGCCATCACCGTCTTCTCGCCCGACGGCCACCTCTTCCAAGTGGAGTACGCGCAGG
AGGCGGTCAAAAAGGGCTCCACCGCGGTTGGTGTTCGAGGAAAGGACATTGTTGTTCTTGGTGTGGAAAA
AAAATCAGTGGCCAAGCTACAAGATGAAAGAACAGTACGAAAAATCTGCGCCTTGGACGATAACGTCTGT
ATGGCCTTTGCAGGTCTCACCGCTGATGCAAGGATAGTCATCAACAGAGCCCGGGTAGAGTGCCAGAGCC
ACCGGCTGACAGTGGAGGACCCAGTGACTGTGGAGTACATCACCCGCTACATTGCGAGTCTGAAGCAGCG
TTATACACAGAGCAATGGGCGCAGGCCATTTGGTATCTCGGCCCTAATTGTGGGTTTTGACTTTGATGGC
ACTCCCAGACTCTATCAGACTGACCCCTCGGGCACATACCATGCTTGGAAGGCCAATGCCATAGGCCGGG
GCGCCAAGTCAGTGCGTGAATTTCTGGAGAAGAACTACACAGATGATGCCATTGAAACAGATGATCTGAC
CATCAAACTTGTGATCAAGGCACTGTTAGAGGTGGTCCAGTCAGGTGGCAAAAACATCGAACTTGCCGTC
ATGAGGCGGGATCAGCCCCTCAAGATTCTAAATCCTGAAGAAATTGAGAAGTATGTTGCTGAAATTGAGA
AGGAGAAAGAAGAAAATGAAAAGAAGAAGCAAAAGAAAGCATCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG203141 representing NM_011969
Red=Cloning site Green=Tags(s)

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGKDIVVLGVEKKSVAKLQDERTVRKICALDDNVC
MAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDG
TPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDDAIETDDLTIKLVIKALLEVVQSGGKNIELAV
MRRDQPLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011969
ORF Size 744 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011969.1, NP_036099.1
RefSeq Size 881 bp
RefSeq ORF 747 bp
Locus ID 26444
UniProt ID Q9Z2U0
Gene Summary Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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