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Styx (NM_019637) Mouse Tagged ORF Clone
CAT＃: MG202580
- TrueORF®
Styx (tGFP-tagged) - Mouse phosphoserine/threonine/tyrosine interaction protein (Styx)
ORF Plasmid: DDK
"NM_019637" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 0610039A20Rik; hStyxb |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202580 representing NM_019637
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGTGAAACTGGAGTTCCCCTCGCTCCCGCAGTGCAAAGACGACGCCGAGGAGTGGACTTACC CTATGAGACGAGAGATGCAGGAAGTTTTGCCTGGATTGTTCTTAGGCCCATACTCTTCTGCTATGAAAAG CAAGCTACCTATACTTCAGAAACATGGCATAACTCATATAATTTGCATACGGCAAAATATTGAAGCAAAC TTTATTAAACCAAACTTTCAACAATTATTTAGATATTTAGTTTTGGATATTGCTGATAATCCAGTTGAAA ACATAATACGTTTTTTCCCTATGACTAAAGAGTTTATTGATGGAAGCTTACAAAATGGAGGAAAAGTTCT TGTCCATGGGAATGCAGGTATCTCTAGAAGTGCTGCCTTTGTCATTGCATACATCATGGAGACGTTTGGG ATGAAATACAGAGATGCATTTGCTTATGTCCAAGAAAGGAGATTTTGTATTAATCCTAATGCTGGATTTG TCCACCAACTTCAGGAATATGAAGCCATCTACTTAGCAAAATTAACAATACAGATGATGTCACCACTCCA AATAGAAAGGTCCTTAGCTGTTCATTCTGGTACCACAGGTAGTGTGAAGAGAACACATGAGGAAGATGAC GACTTTGGAAACATGCAAGTGGCAACCGCACAGAATGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202580 representing NM_019637
Red=Cloning site Green=Tags(s) MEDVKLEFPSLPQCKDDAEEWTYPMRREMQEVLPGLFLGPYSSAMKSKLPILQKHGITHIICIRQNIEAN FIKPNFQQLFRYLVLDIADNPVENIIRFFPMTKEFIDGSLQNGGKVLVHGNAGISRSAAFVIAYIMETFG MKYRDAFAYVQERRFCINPNAGFVHQLQEYEAIYLAKLTIQMMSPLQIERSLAVHSGTTGSVKRTHEEDD DFGNMQVATAQNG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019637 |
| ORF Size | 669 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019637.3, NP_062611.2 |
| RefSeq Size | 4258 bp |
| RefSeq ORF | 672 bp |
| Locus ID | 56291 |
| UniProt ID | Q60969 |
| Gene Summary | Catalytically inactive phosphatase (PubMed:7592916). Acts as a nuclear anchor for MAPK1/MAPK3 (ERK1/ERK2) (By similarity). Modulates cell-fate decisions and cell migration by spatiotemporal regulation of MAPK1/MAPK3 (ERK1/ERK2) (By similarity). By binding to the F-box of FBXW7, prevents the assembly of FBXW7 into the SCF E3 ubiquitin-protein ligase complex, and thereby inhibits degradation of its substrates (By similarity). Plays a role in spermatogenesis (PubMed:11842224).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203235 | Styx (untagged) - Mouse serine/threonine/tyrosine interaction protein (Styx), (10ug) |
CNY 2,000.00 |
|
| MR202580 | Styx (Myc-DDK-tagged) - Mouse serine/threonine/tyrosine interaction protein (Styx) |
CNY 2,081.00 |
|
| MR202580L3 | Lenti ORF clone of Styx (Myc-DDK-tagged) - Mouse serine/threonine/tyrosine interaction protein (Styx) |
CNY 4,750.00 |
|
| MR202580L4 | Lenti ORF clone of Styx (mGFP-tagged) - Mouse serine/threonine/tyrosine interaction protein (Styx) |
CNY 4,750.00 |
