Taf10 (NM_020024) Mouse Tagged ORF Clone

CAT#: MG202464

  • TrueORF®

Taf10 (tGFP-tagged) - Mouse TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf10)

ORF Plasmid: DDK tGFP



  "NM_020024" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 30kDa; AU041226; Taf2h; TAFII30
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG202464 representing NM_020024
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGCAGCGGCTCCGGCGCGGACCCCGAGGCGGCGACCGCCTGTGCCGCCTCGGTCGCGGGTCCGG
CACCCCTGGTGTCGGCTCCCGCTGCGCTGCCCACTAGCACGGCCGCGGAGAGCAAGGCCAGCCCCGCGGG
GACAGCAGGGGGCCCCGTGGCTGGAGTCGCCACTGCGGGCACGGGCCCCGTGGCGGCACGGGCAGGGGAG
CCGGCAGAGCGGCGCGGGCCAGCTTCGGTGGCAGCGGGCGGCGCGGCTCCCCCGGAGGGGGCAATGTCTA
ACGGGGTTTACGCCCTACCGAGCGCGGCCAACGGAGAAGTGAAGCCCGTAGTGTCCAGCACACCTCTAGT
GGACTTCTTGATGCAGTTGGAGGATTACACACCTACGATCCCAGATGCAGTGACTGGTTACTACCTGAAC
CGCGCTGGCTTTGAAGCTTCAGACCCACGCATAATTCGGCTCATCTCACTAGCTGCCCAGAAATTCATCT
CAGATATTGCCAATGATGCCCTACAGCACTGCAAAATGAAGGGCACAGCCTCTGGCAGCTCCCGGAGCAA
GAGCAAGGATCGCAAGTACACCCTAACCATGGAGGACTTGACCCCTGCCCTCAGCGAGTATGGCATCAAT
GTGAAGAAGCCGCACTACTTCACC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG202464 representing NM_020024
Red=Cloning site Green=Tags(s)

MSCSGSGADPEAATACAASVAGPAPLVSAPAALPTSTAAESKASPAGTAGGPVAGVATAGTGPVAARAGE
PAERRGPASVAAGGAAPPEGAMSNGVYALPSAANGEVKPVVSSTPLVDFLMQLEDYTPTIPDAVTGYYLN
RAGFEASDPRIIRLISLAAQKFISDIANDALQHCKMKGTASGSSRSKSKDRKYTLTMEDLTPALSEYGIN
VKKPHYFT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020024
ORF Size 654 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020024.3, NP_064408.2
RefSeq Size 787 bp
RefSeq ORF 657 bp
Locus ID 24075
UniProt ID Q8K0H5
Gene Summary TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May regulate cyclin E expression.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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