Uba1 (NM_001276317) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A1; A1S9; Sb; Sbx; Ube-1; Ube1x
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229323 representing NM_001276317 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCT CCCCTGCACAGTCTGCGCTGTCCGAAGTGTCCTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGA AGCAGACATAGACGAGAGCCTTTACTCCCGGCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTC CAGACATCCAGCGTCCTTGTCTCAGGCTTGCGGGGCTTGGGTGTAGAAATTGCTAAGAACATCATCCTTG GTGGGGTCAAGGCTGTCACCCTACATGACCAAGGAACTACCCAGTGGGCTGATCTCTCCTCCCAGTTTTA CCTTCGGGAGGAGGACATTGGTAAAAATCGAGCGGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGC TATGTACCTGTCACTGCCTACACTGGGCCTCTTGTCGAGGACTTCCTTAGTAGCTTCCAGGTGGTGGTCC TCACCAACAGCCCCCTGGAAGCCCAGCTGCGAGTGGGGGAGTTCTGTCATAGCCGTGGTATCAAGCTAGT GGTGGCAGATACAAGAGGCCTGTTTGGGCAACTTTTCTGTGATTTTGGAGAGGAAATGGTCCTCACAGAT TCCAATGGGGAGCAGCCACTCAGTGCTATGGTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCT GCCTTGATGAGGCCCGACATGGCTTTGAGACTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGAT CCAACTCAATGGATGTCAGCCCATGGAGATCAAAGTGCTGGGTCCTTATACCTTTAGTATCTGTGACACT TCCAACTTCTCTGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCGAAAAAGATTAGTTTTA AATCCTTGCCAGCATCACTGGTAGAGCCTGACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGC CCAACTGCACATTGGCTTCCAAGCTCTGCACCAATTCTGTGCTCTGCACAACCAACCACCTCGACCACGA AATGAGGAAGATGCAACAGAGCTGGTGGGCCTGGCTCAGGCTGTAAACGCTCGGTCCCCACCTTCAGTAA AACAGAACAGCTTGGATGAAGACCTTATTCGGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCCAT AAATGCTTTCATTGGGGGCCTTGCTGCCCAGGAAGTCATGAAGGCCTGCTCTGGAAAGTTTATGCCCATC ATGCAGTGGTTGTACTTTGATGCTCTTGAATGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGAGAAGT GCCTCCCACGTCAGAACCGTTACGATGGGCAGGTAGCTGTATTTGGGTCAGACTTTCAGGAGAAGCTGAG CAAGCAAAAGTACTTCCTGGTGGGTGCAGGGGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATT GGGCTGGGTTGTGGAGAGGGTGGAGAGGTCGTGGTCACAGACATGGACACCATTGAGAAATCAAATCTGA ACCGACAGTTTCTCTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCG CCAGATGAATCCTTACATCCAGGTGACAAGCCACCAGAACCGTGTAGGTCCTGACACTGAGCGCATCTAT GATGATGATTTCTTCCAAAATTTGGATGGTGTGGCCAATGCTCTGGACAACATAGATGCCCGCATGTACA TGGATCGCCGATGTGTGTACTATCGGAAGCCACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAACGT GCAGGTGGTAATCCCCTTCCTGACAGAATCCTACAGCTCTAGCCAGGATCCACCAGAGAAATCCATCCCC ATTTGTACCCTGAAAAACTTTCCCAATGCCATCGAACACACTCTTCAGTGGGCCCGGGATGAATTTGAAG GCCTTTTCAAGCAGCCAGCAGAAAATGTTAATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATT GCGGCTGGCTGGTACCCAGCCATTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGTTGCAGCGACCA CAGACTTGGGGAGACTGTGTGACCTGGGCCTGCCACCACTGGCACACCCAGTACTGTAACAACATCCGGC AACTGCTGCACAACTTTCCTCCTGACCAGCTCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACG CTGTCCACACCCACTTACTTTTGATGTTAACAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAAC CTTTTTGCCCAGACCTATGGGTTGACTGGATCCCAAGACCGAGCTGCTGTGGCCTCACTCCTGCAGTCAG TACAAGTCCCAGAGTTCACCCCCAAGTCTGGTGTCAAGATTCATGTTTCTGACCAGGAGTTGCAGAGTGC CAATGCCTCTGTTGATGACAGCCGTCTTGAGGAGCTCAAAGCCACATTGCCCAGCCCAGACAAGTTACCC GGATTTAAGATGTACCCCATTGATTTTGAGAAGGATGATGACAGCAATTTCCACATGGATTTCATTGTGG CTGCATCCAATCTTCGGGCCGAAAACTATGATATTTCCCCTGCAGACCGACACAAGAGCAAGCTGATTGC AGGGAAGATCATCCCAGCCATTGCCACAACCACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAGCTCTAC AAGGTAGTTCAAGGGCACCAACAGCTCGATTCCTACAAAAATGGTTTCCTGAACTTGGCCCTGCCCTTCT TTGGGTTTTCTGAACCTCTTGCTGCACCTCGTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCG CTTTGAAGTACAAGGGCTGCAGCCTAATGGTGAGGAGATGACCCTCAAGCAGTTCCTTGATTACTTTAAG ACAGAGCACAAATTGGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCTTTCTTCATGCCAG CTGCTAAGCTCAAGGAACGATTGGATCAGCCGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCT GGGCCGCCATGTGCGGGCACTGGTGCTTGAGCTGTGCTGCAACGATGAAAGCGGCGAGGACGTCGAGGTC CCTTATGTCCGATATACCATTCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276317
Insert Size	3177 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276317.1, NP_001263246.1
RefSeq Size	4068 bp
RefSeq ORF	3177 bp
Locus ID	22201
UniProt ID	Q02053
Gene Summary	This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 3. Variants 2, 3, and 4 encode the same isoform (2).

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