Prlr (NM_001253781) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI987712; Pr-1; Pr-3; Prlr-rs1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226951 representing NM_001253781 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTGCACTTGCTTACATGCTGCTTGTCCTCAGCATCAGCCTCCTGAATGGACAGTCACCACCTG GAAAACCTGAAATCCACAAATGTCGTTCCCCTGACAAGGAAACATTCACCTGCTGGTGGAATCCTGGGTC AGATGGAGGACTCCCCACCAATTATTCATTGACATACAGCAAAGAAGGAGAGAAAAACACCTATGAATGT CCAGACTACAAAACCAGTGGCCCCAATTCCTGTTTCTTTAGCAAGCAGTACACTTCCATATGGAAAATAT ACATCATCACAGTAAATGCCACGAACGAAATGGGAAGCAGTACCTCGGATCCACTTTATGTGGATGTGAC TTACATTGTTGAACCAGAGCCTCCTCGGAACCTGACTTTAGAAGTGAAACAACTAAAAGACAAAAAAACA TATCTGTGGGTAAAATGGTTGCCACCTACCATAACTGATGTAAAAACTGGTTGGTTTACAATGGAATATG AAATTCGATTAAAGTCTGAAGAAGCAGATGAGTGGGAGATCCACTTCACAGGTCATCAAACACAATTTAA GGTTTTTGACTTATATCCAGGACAAAAGTATCTTGTCCAGACTCGCTGCAAGCCAGACCATGGATACTGG AGTAGATGGGGCCAGGAGAAATCTATTGAAATACCAAATGACTTCACCTTGAAAGACACAACTGTGTGGA TCATTGTGGCCGTTCTCTCTGCTGTCATCTGTTTGATTATGGTCTGGGCAGTGGCTTTGAAGGGTTATAG CATGATGACCTGCATCTTTCCACCAGTTCCGGGGCCAAAAATAAAAGGATTTGATACTCATCTGCTAGAG ACTGGCTCTCCAAGCAAATACAAGGTGGATCCACGCCTGGCCCTTCCTGGACGATTCCAAAAACTGGACA ATGAAGGGGAACTTGACTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253781
Insert Size	933 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253781.1, NP_001240710.1
RefSeq Size	3916 bp
RefSeq ORF	933 bp
Locus ID	19116
Gene Summary	This is a receptor for the anterior pituitary hormone prolactin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate 5' UTR exon and an alternate 3' exon including the coding region and UTR, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.

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