Elane (NM_015779) Mouse Untagged Clone

CAT#: MC209856

Elane (untagged) - Mouse elastase, neutrophil expressed (Elane), (10ug)



  "NM_015779" in other vectors (4)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms El; Ela2; F430011M15Rik; N; NE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_015779, the custom clone sequence may differ by one or more nucleotides


ATGGCCCTTGGCAGACTATCCAGCCGGACTCTGGCTGCCATGCTACTGGCATTGTTCCTGGGTGGCCCAG
CACTGGCCTCAGAGATTGTTGGTGGCCGGCCGGCCCGGCCCCATGCTTGGCCCTTCATGGCATCCCTGCA
GAGGCGTGGAGGTCATTTCTGTGGTGCCACCCTCATTGCCAGGAACTTCGTCATGTCAGCAGCCCACTGT
GTGAACGGCCTAAATTTCCGGTCAGTGCAGGTAGTGCTGGGAGCCCATGACCTGCGGCGACAGGAGCGCA
CTCGACAGACCTTCTCTGTGCAGCGGATCTTCGAGAATGGCTTTGACCCATCACAACTGCTGAACGACAT
TGTGATTATCCAGCTCAATGGCTCCGCTACCATTAACGCCAACGTGCAGGTGGCCCAGCTGCCTGCCCAG
GGCCAGGGCGTGGGTGACAGAACTCCATGTCTGGCCATGGGCTGGGGCAGGTTGGGCACAAACAGACCAT
CACCCAGTGTGCTACAAGAGCTCAATGTGACAGTGGTGACTAACATGTGCCGCCGTCGTGTGAACGTATG
CACTCTGGTGCCACGTCGGCAGGCAGGCATCTGCTTCGGGGACTCTGGCGGACCCTTGGTCTGTAACAAC
CTTGTCCAAGGCATTGACTCCTTCATCCGAGGAGGCTGTGGATCTGGATTGTACCCAGATGCCTTCGCCC
CTGTGGCTGAGTTTGCAGATTGGATCAATTCCATTATCCGAAGCCATAATGACCACCTTCTTACCCATCC
CAAAGACCGAGAGGGCAGGACCAACTAG


Restriction Sites SgfI-MluI     
ACCN NM_015779
Insert Size 798 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC145800, AAI45801
RefSeq Size 905 bp
RefSeq ORF 798 bp
Locus ID 50701
UniProt ID Q3UP87
Gene Summary This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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