Akr1b3 (BC004725) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Akr1b1, AR, ALR2
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004725 CGGACGCGTGGGTTTGTAACGTGCAGCGATCATGGCCAGCCATCTGGAACTCAACAACGGCACCAAGATG CCCACCCTGGGCCTGGGCACCTGGAAGTCCCCTCCTGGCCAGGTGACTGAGGCCGTGAAAGTTGCTATTG ACTTGGGGTACCGCCACATTGACTGCGCCCAGGTGTACCAGAATGAGAAGGAGGTGGGAGTGGCCCTCCA GGAGAAGCTCAAGGAGCAGGTGGTGAAGCGGCAGGATCTCTTCATTGTCAGCAAGCTGTGGTGCACGTTC CATGACAAGAGCATGGTGAAAGGAGCCTTCCAGAAGACACTGAGCGACCTGCAGCTGGACTACCTGGATC TCTACCTTATTCACTGGCCAACGGGGTTCAAGCCTGGGCCCGACTATTTCCCACTGGATGCCTCAGGGAA CGTGATACCTAGTGACACCGATTTTGTGGACACTTGGACGGCTATGGAACAACTAGTGGATGAAGGTTTG GTGAAAACAATCGGTGTCTCCAACTTCAACCCTCTTCAGATTGAGAGGATCTTGAACAAACCTGGCTTAA AATATAAGCCTGCGGTGAACCAGATCGAGTGCCACCCGTACCTAACTCAGGAGAAGCTGATTGAATACTG CCACAGCAAAGGCATCGTGGTGACAGCATATAGTCCCCTTGGTTCTCCTGACAGGCCCTGGGCCAAGCCT GAAGATCCGTCTCTCCTGGAGGATCCCAGGATCAAAGCAATTGCAGCCAAGTACAATAAAACTACAGCCC AGGTGCTGATCCGGTTCCCCATTCAGAGGAACTTGGTAGTGATCCCCAAGTCTGTGACACCAGTGCGCAT TGCTGAGAACTTGAAGGTCTTTGACTTTGAGCTGAGCAGCGAGGACATGGCCACTCTACTCAGCTACAAC AGGAACTGGAGGGTGTGCGCCTTGATGAGCTGTGCCAAACACAAGGATTACCCCTTCCACGCCGAAGTCT GAAGCTGTGGCGAGCGGATCCCTGCTCCTCCTCAAGTGGCTTGCACCTGTTCTTTCTGCCTCATCTGCCC TTGCAAGTGTCCTGTGTCTGCACTAGGTGGCACCTTGCAAACCAGATGGTGAGAGTTAGTTAGTTTGACA TAGAGTCTGGAGGGCAGTACCAGTAGCCAAGGTGTTTCTTCCAGCCTTCCTTGGTCTTTCTTCTTACCCA CCTGGAGGACTTTAACACAAGTACCCTTTCCAACCAAAGAGAAGCAAGCTTTAGAGTCCAAGCCGTGCCA CTAACACTTAAATGTGAGTGCTTAGAACTGTAATCCTTTGGGTCAGACTTCTCCTTGCCTCAAATAAAAA CTGCTTTTGTGAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC004725
Insert Size	951 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC004725, AAH04725
RefSeq Size	1358 bp
RefSeq ORF	951 bp
Locus ID	11677
Gene Summary	Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed:17381426, PubMed:19010934, PubMed:7851421). Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins (PubMed:19010934). Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (By similarity).[UniProtKB/Swiss-Prot Function]

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