AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.A2.B2.H9]

Specifications

Product Data
Clone Name	14E5.A2.B2.H9
Applications	WB
Recommend Dilution	WB: User Optimized
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT3 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	See kit insert for complete instructions.
Database Link	UniProt ID P31749
Background	Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for AKT1 combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of native and/or recombinant human AKT1 protein. AKT1 Western Blotting kit allows for the detection of endogenous protein levels of human AKT1 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT1. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT1 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT1 Western Blotting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.
Synonyms	AKT 1 western blotting, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, Chemiluminescent WB, Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit
Note	Use Rockland Immunochemicals’ Anti-AKT1 Chemiluminescent Kit for Western Blotting for detection of human AKT1 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT1 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.