AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.16C8.25F6]

Specifications

Product Data
Clone Name	14E5.16C8.25F6
Applications	ELISA, FC, IHC, WB
Recommend Dilution	WB: 1:500 - 1:3,000 IHC: 20 ug/ml FC: User Optimized ELISA: 1:20,000
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Anti-Akt monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human AKT1 protein.
Specificity	This antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein.  A BLAST analysis was used to suggest cross-reactivity with AKT from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence.  Cross-reactivity with AKT from other sources has not been determined.  Cross-reactivity with AKT2 and AKT3 has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store Akt Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Gene Name	AKT serine/threonine kinase 1
Database Link	UniProt ID P31749
Background	AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Synonyms	mouse anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Note	Anti-AKT monoclonal antibody has been tested by ELISA and western blotting. This antibody is suitable in immunohistochemistry and immunoprecipitation. Expect a band approximately 56 kDa in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human and mouse AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.
Reference Data

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