CD31 (PECAM1) Rat Monoclonal Antibody [Clone ID: MEC13.3]

Specifications

Product Data
Clone Name	MEC13.3
Applications	CyTOF-ready, FC, ICC/IF, IHC, IP
Recommend Dilution	Immunohistochemistry-Frozen: 1:100 , Immunohistochemistry: 1:100-1:500, Immunoprecipitation: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:500 - 1:1000, Flow Cytometry: 2.5 ug/ml, Flow (Cell Surface): 2.5 ug/ml, In vivo assay, CyTOF-ready
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	mouse endothelial cell line T-end
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	platelet and endothelial cell adhesion molecule 1
Database Link	NP_000433 Entrez Gene 18613 Mouse UniProt ID P16284
Background	CD31/PECAM1 is commonly used as an endothelial marker, particularly in angiogenesis. CD31 is important in assisting placental and endometrial vascular development. However, CD31 is also associated with many negative angiogenic occurrences, particularly those within or related to tumors and other disease lesions. In MS, plasma microparticules carry CD31 and are important in the formation of brain lesions associated with MS. In cardiac angiosarcoma, CD31 presence is indicative of the severity of the disease. In prostate tumor cells, CD31 is upregulated and associated with increased levels of HIF1a, VEGF, and other hypoxic markers. This relationship seems to indicate that when prostate cancer reaches a sufficient state of development that intense angiogenesis must occur in order to support the ongoing growth and survival of the cancer cells. In all, CD31 is a great marker for normal and disease-induced angiogenesis.
Synonyms	CD31; EndoCAM; endoCAM; GPIIA; PECA1; PECAM-1
Note	This CD31/PECAM1 Antibody (MEC13.3) is useful for Flow Cytometry, Immunoprecipitation, Immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and in vitro and in vivo blocking of CD31-mediated cell-cell interactions. Please Note: NB600-1475 works well on acetone-fixed frozen sections as well as zinc-fixed paraffin-embedded sections. However, it is not recommended for formalin-fixed paraffin-embedded sections due to inconsistent results. In vivo assay was reported in scientific literature. Use in vitro assays reported in scientific literature (PMID 24647208)
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Leukocyte transendothelial migration

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