Chk2 (CHEK2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 200-1000 WB positive control: MCF-7, 293T, HT-29, Hela and Jurkat cells
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a region derived from 2-18 amino acids of Human Checkpoint kinase 2
Formulation	PBS pH7.3, 0.05% NaN3, 50% glycerol
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	61 kDa
Gene Name	checkpoint kinase 2
Database Link	NP_001005735 Entrez Gene 11200 Human UniProt ID O96017
Background	In response to DNA damage and replication blocks; cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated; the encoded protein is known to inhibit CDC25C phosphatase; preventing entry into mitosis; and has been shown to stabilize the tumor suppressor protein p53; leading to cell cycle arrest in G1. In addition; this protein interacts with and phosphorylates BRCA1; allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome; a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also; mutations in this gene are thought to confer a predisposition to sarcomas; breast cancer; and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Synonyms	CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways	Cell cycle, p53 signaling pathway

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