AIF (AIFM1) (517-531) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 0.25-1 µg/ml. Immunohistochemistry on Paraffin Sections: 10 µg/ml; this product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH6.0 is recommended for this purpose.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide corresponding to amino acids 517 - 531 of human AIF
Specificity	This antibody recognises Apoptosis Inducing Factor (AIF). The antibody reacts with an internal region of AIF.
Formulation	PBS containing 0.02% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Ion Exchange Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	apoptosis inducing factor, mitochondria associated 1
Database Link	Entrez Gene 9131 Human UniProt ID O95831
Background	Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.
Synonyms	PDCD8
Reference Data

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