TIE2 (TEK) Mouse Monoclonal Antibody [Clone ID: Cl.16]

Specifications

Product Data
Clone Name	Cl.16
Applications	ELISA, FC, IHC, WB
Recommend Dilution	ELISA: Use at 1-15 µg/ml. Western blotting: Use at 1-2 µg/ml. Flow Cytometry and Cell Sorting (FACS): Use at 2-5 µg/ml together with the appropriate secondary reagents.  Immunohistochemistry: Use at 1-5 μg/ml (Cryo).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human soluble extracellular domain of TIE-2 protein
Specificity	The monoclonal antibody will detect native Human TIE-2/tek in ELISA experiments and on the surface of different cell types.
Formulation	PBS, pH 7.4, without preservatives State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatoghraphy on Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	TEK receptor tyrosine kinase
Database Link	Entrez Gene 7010 Human UniProt ID Q02763
Background	TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.
Synonyms	TIE2, TIE-2, Angiopoietin-1 receptor, p140 TEK
Reference Data

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