HSP70-1A (HSPA1A) Mouse Monoclonal Antibody [Clone ID: OTI2C12]

Specifications

Product Data
Clone Name	OTI2C12
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:50, IF 1:100
Reactivity	Human, Dog, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	70.0 kDa
Gene Name	heat shock protein family A (Hsp70) member 1A
Database Link	NP_005336 Entrez Gene 24472 RatEntrez Gene 193740 MouseEntrez Gene 3303 Human UniProt ID P0DMV8
Background	This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Synonyms	HEL-S-103; HSP70-1; HSP70-1A; HSP70.1; HSP70I; HSP72; HSPA1
Reference Data
Protein Pathways	Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Prion diseases, Spliceosome

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