PRNP Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western Blot: 1/2500.  Immunohistochemistry: 1/1000-1/5000. Quality Control: Antibodies were analyzed by Immunocytochemistry (1/1000 dilution) using cells transfected with the prion protein cDNA.
Reactivity	Human, Mouse, Rat
Host	Chicken
Clonality	Polyclonal
Immunogen	Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund’s complete and incomplete adjuvants. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached. Finally, affinity purified antibodies (50 ug/ml final concentration) were mixed with the IgY fraction (10 mg/ml final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% Sodium Azide.
Formulation	0.9% (w/v) Sodium Phosphate (10 mM, pH 7.2) buffered isotonic saline, 50% (v/v) Glycerol, with  0.02% (w/v) Sodium Azide as an anti-microbial agent State: Purified State: Mixture of IgY fraction (10 mg/ml) and affinity-purified antibodies (50 µg/ml)
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted  (in aliquots) at -20°C. This products is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Database Link	UniProt ID P23907
Background	Prion protein is the protein responsible for human Creutzfeldt-Jakob Disease (CJD), and somatic mutations of the prion gene are responsible for Gerstmann-Straussler-Scheinker disease and Fatal Familial Insomnia. The bovine homolog of the prion protein is responsible for bovine Spongioform Encephalopathy (BSE), also known as "Mad Cow Disease." In all cases, the prion protein has two folding states, with one state being the "normal" non-pathological form, and the other state inducing a spongioform encephalopathy.
Synonyms	Major prion protein, PrP27-30, PrP33-35C, ASCR, PRNP, PRIP
Reference Data

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