MAPKAP Kinase 2 (MAPKAPK2) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Peptide ELISA: 1/128000 (Detection Limit). Western blot: 0.5-2 μg/ml. Approx 60kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kDa according to Mouse NP_032577.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Menon et al, Cell Motil Cytoskeleton. 2009 Dec;66(12):1041-7. PMID: 19743408). There is no cross-reactivity to MK5. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 µg AP32810PU-N or AP32811PU-N. Western blots of the Immunoprecipitation were labelled with rabbit anti-MK2 (CST). An approx 45kDa of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunohistochemistry on Paraffin Sections: 5 µg/ml in paraffin embedded Human Heart.
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_004750.1; NP_116584.2.
Specificity	This antibody is expected to recognize both reported isoforms (NP_004750.1; NP_116584.2).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase-activated protein kinase 2
Database Link	Entrez Gene 9261 Human UniProt ID P49137
Synonyms	MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MK2, MAPKAPK2
Reference Data

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