CNPase (CNP) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemstry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. 1/2000-1/5000 for Western blots. Quality Control: Quality assurance analysis was performed using Immunohistochemistry (at a dilution of 1/2000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Synthetic peptide KLH conjugated corresponded to a region of the CNPase gene product shared between the Human (NP_149124) and Mouse (P16330) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.
Specificity	Recognizes 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase (CNPase).
Formulation	10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction.
Concentration	lot specific
Purification	Affinity Chromatography using a peptide column.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted in the dark at 2-8°C.
Gene Name	2',3'-cyclic nucleotide 3' phosphodiesterase
Database Link	Entrez Gene 12799 MouseEntrez Gene 1267 Human UniProt ID P09543
Background	Murine CNPase (EC 3.1.4.37) is a 47,122 dalton protein (420 amino acids) found in myelin. This protein is expressed by oligodendrocytes of the CNS and by Schwann cells of the PNS.
Synonyms	CNP, CNP1, Oligodendrocyte Marker
Reference Data

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