EGF Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on paraffin embedded tissue: 1/50 - 1/200. Western blot: 1/500 - 1/1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of EGF protein. (region surrounding Arg709)
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity chromatography using epitope-specific immunogen (> 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 130 kDa
Gene Name	epidermal growth factor
Database Link	Entrez Gene 13645 MouseEntrez Gene 25313 RatEntrez Gene 1950 Human UniProt ID P01133
Background	Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein of 6 kDa originally found in rodents and humans. EGF has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. It binds to the 170 kDa EGF receptor on the surface of cells and mediates intrinsic phos phoryl ation of the receptor on tyrosine residues. EGF has been detected in nearly all body fluids such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF, TGFalpha and vaccinia virus growth factor exhibit 30-40% amino acid homology. Several additional members of the EGF/TGF family have been described; these include Cripto, Amphiregulin and the heparin-binding EGF-like growth factor, each of which, except Cripto, binds to the EGF receptor.
Synonyms	Urogastrone, Epidermal growth factor, URG, HOMG4
Reference Data
Protein Families	Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
Protein Pathways	Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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