14 3 3 gamma (YWHAG) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 50-100 of Human 14-3-3 γ.
Specificity	This antibody detects endogenous levels of 14-3-3 gamma protein. (region surrounding Met81)
Formulation	Phosphate Buffered Saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~28 kDa
Gene Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
Database Link	Entrez Gene 22628 MouseEntrez Gene 56010 RatEntrez Gene 7532 Human UniProt ID P61981
Background	14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, epsilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
Synonyms	YWHAG, KCIP-1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis

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