Col3a1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC
Recommend Dilution	Immunohistochemistry on frozen sections. ELISA: 1/1500 - 1/6000. Immunofluorescence.
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagen type III from rat tail tendon
Specificity	This antibody is specific for rat Collagen 3 binding to both native and heat denatured collagen 3. This antibody is reactive with rat kidney, liver, skin and heart, but does not stain basement membranes.
Formulation	Contains mannitol, dextran and salts State: Purified State: Lyophilised purified IgG
Reconstitution Method	Reconstitute in 0.5 ml of distilled water.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	collagen, type III, alpha 1
Database Link	Entrez Gene 84032 Rat UniProt ID P13941
Background	Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Synonyms	COL3A1
Reference Data

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