VEGF Receptor 2 (KDR) Rat Monoclonal Antibody [Clone ID: 3C8]

Specifications

Product Data
Clone Name	3C8
Applications	FC, IF, WB
Recommend Dilution	Western Blot: 2-5 µg/ml. Immunofluorescence: 2-10 µg/ml. FACS: 1-5 µg/ml.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Purified, Insect Cell-derived recombinant Mouse soluble FLK1/KDR (110kDa).
Specificity	This antibody will detect native Human VEGFR-2/KDR on the surface of different Human cell types.
Formulation	PBS pH 7.4 without preservatives State: Purified State: Lyophilized purified IgG fraction Stabilizer: None
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	kinase insert domain receptor
Database Link	UniProt ID P35968
Background	VEGF R2 (mouse Flk1 gene), VEGF R1 (Flt1) and VEGF R3 (Flt4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulinlike repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGF R2 cDNA encodes a 1367 amino acid (aa) precursor protein with a 19 aa signal peptide. Mature VEGF R2 is composed of a 743 aa extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The solube receptor was used as a an antigen.
Synonyms	VEGFR2, FLK1, KDR, VEGF Receptor 2
Reference Data

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