GFAP Mouse Monoclonal Antibody [Clone ID: SPM507]

Specifications

Product Data
Clone Name	SPM507
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5 µg/106 cells in 0.1 ml.  Immunofluorescence: 1-2 µg/ml. Western Blot: 0.5-1 µg/ml.  Immunoprecipitation: 1-2 μg/500 µg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Brain or Astrocytoma.
Reactivity	Bovine, Chicken, Human, Mouse, Porcine, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	GFAP isolated from pig spinal cord.
Specificity	This Monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	~50 kDa
Gene Name	glial fibrillary acidic protein
Database Link	Entrez Gene 2670 Human UniProt ID P14136
Background	Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system.
Synonyms	Glial Fibrillary Acidic Protein
Reference Data

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