Egf Mouse Monoclonal Antibody [Clone ID: F5]

Specifications

Product Data
Clone Name	F5
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA.  Spot Blots.  Immunohistochemistry on Fixed Frozen Sections: 1/20.  Immunohistochemistry on Paraffin Sections of Salivary glands (see Protocols for more details.)
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	EGF coupled on BSA and tetanus toxoide
Specificity	The antibody reacts with Mouse EGF in ELISA (10 ng detectable) and in Spot Blots (1 ng detectable).  In Immunohistochemistry the antibody reacts with formalin fixed and paraffin embedded Mouse salivary glands. It also reacts with Human Brunner's glands (presumably with urogastron).
Formulation	State: Supernatant State: Tissue Culture Supernatant Stabilizer: 1.0% BSA Preservative: 20 mM Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.  Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor
Database Link	Entrez Gene 13645 Mouse UniProt ID P01132
Background	Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein.  Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
Synonyms	Urogastrone, Epidermal growth factor, URG, HOMG4
Reference Data

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