EIF4E Mouse Monoclonal Antibody [Clone ID: 5D11]

Specifications

Product Data
Clone Name	5D11
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500 - 1/2000. Flow Cytometry: 1/200 - 1/400. Immunofluorescence: 1/200 - 1/1000. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Human EIF4E expressed in E. Coli.
Specificity	Recognizes EIF4E
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	25 kDa
Gene Name	eukaryotic translation initiation factor 4E
Database Link	Entrez Gene 1977 Human UniProt ID P06730
Background	eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
Synonyms	eIF-4F 25 kDa subunit, EIF-4E, EIF4EL1, EIF4F
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.