OriGene Technologies, Inc.
  邮箱:  密码: 忘记密码?
注册 | 购物车 | 主页
搜索:    
About FL cDNA Clones RNAi Gene Expression Lysates and Proteins Antibodies Tissue IVD 原料 Other Products Tech Support
orange
按通路检索:
按应用检索:
按蛋白家族检索:
按产品线检索:
按抗体类型检索:
按应用种属检索:
按宿主来源检索:
TrueMABTM Antibodies - Made against Authentic Protein Antigens

Anti-TUBB3 TRUEMAB Antibody Clone OTI5H2

div

Specifications Related Products Product Reviews FAQs
Catalog No. Description Amount Price Shipping
TA500047
  • TUBB3 (beta III Tubulin) mouse monoclonal antibody, clone OTI5H2 (formerly 5H2)
100ul 2500 现货 Add to Shopping Cart
TA500047S
  • TUBB3 (beta III Tubulin) mouse monoclonal antibody, clone OTI5H2 (formerly 5H2)
30ul850现货Add to Shopping Cart
CF500047
  • TUBB3 (beta III Tubulin) mouse monoclonal antibody, clone OTI5H2 (formerly 5H2)
100 ug4275.00现货Add to Shopping Cart
   TrueMABTM Antibodies
* Delivery is in business days
WB(3)
Also for TUBB3 (Locus ID 10381)
Gene NameHomo sapiens tubulin beta 3 class III (TUBB3), transcript variant 1 Synonyms:beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4
ImmunogenHuman recombinant protein fragment corresponding to amino acids 1-150 of human TUBB3 (NP_006077) produced in E.coli.
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone OTI5H2 IsotypeIgG1
Species ReactivityHuman Concentration0.45 mg/ml
Purification Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *WB Suggested DilutionsWB 1:1000~2000,
BackgroundTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules.
Related Pathway
Cell cycle

* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB3 (RC200755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB3.
spacer

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBB3 monoclonal antibody at 1:200. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
spacer

WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TUBB3-Knockout Hela cells (KO, Cat# LC810307) were separated by SDS-PAGE and immunoblotted with anti-TUBB3 monoclonal antibody TA500047. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.(1:500)
spacer



 

spacer
全国免费电话:400-6744-363(手机),800-9904-363(座机)订货邮箱:orderscn@origene.com,techsupport@origene.cn
电话:+86-10-59755312 传真:+86-10-59755322-888 地址:北京市经济技术开发区科创14街99号汇龙森科技园11栋
京ICP证07031721号 Copyright © 2017 北京傲锐东源(OriGene Technologies)生物科技有限公司,All Rights Reserved. 法律声明.