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ERCC1 UltraMAB Antibody, Clone 4F9

UltraMAB? Antibodies - Validated with 10K Protein Chip


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SKU Description Datasheet Price Availability*  
  • anti-ERCC1 mouse monoclonal antibody, clone 4F9, 100 ul
4446 现货 Add to Shopping Cart
  • anti-ERCC1 mouse monoclonal antibody, clone 4F9, 30 ul
¥1140 现货 Add to Shopping Cart
  • Carrier-free (BSA/glycerol-free) anti-ERCC1 mouse monoclonal antibody, clone 4F9
¥5016 现货 Add to Shopping Cart
Also for ERCC1 (NM_001983)
Gene NameHomo sapiens ERCC excision repair 1, endonuclease non-catalytic subunit (ERCC1), transcript variant 2
SynonymsCOFS4; RAD10; UV20
ImmunogenProtein expressed in 293T cell transfected with human ERCC1 expression vector
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone 4F9 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100,
BackgroundThe product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (Clone 4F9).
WB Image
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and ERCC1-Knockout Hela cells (KO, Cat# LC810072) were separated by SDS-PAGE and immunoblotted with anti-ERCC1 monoclonal antibody UM500008. Then the blotted membrane was stripped and reprobed with?anti-HSP90AB1 antibody (TA500494) as a loading control.(1:500)
IHC Image
Immunohistochemical staining of FFPE normal adjacent lung from tumor specimen using heat-induced epitope retrieval HIER at 120C for 3min with Accel buffer pH8.7, mouse monoclonal antibody anti-ERCC1 clone 4F9 was used at 1ug/mL. Strong nuclear stain in lung pneumocytes and macrophages.
IHC Image
Immunohistochemical staining of FFPE endometrial carcinoma using heat-induced epitope retrieval HIER at 120C for 3min with Accel buffer pH8.7, mouse monoclonal antibody anti-ERCC1 clone 4F9 was used at 1ug/mL. Strong nuclear stain in tumor cells.
IF Image
Immunofluorescent staining of HeLa cells using anti-ERCC1 mouse monoclonal antibody (UM500008, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20?m.
10K-CHIP Image
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERCC1 mouse monoclonal antibody (Clone 4F9). The positive reactive proteins are highlighted with red arrows in the enlarged subarray. Other positive controls spotted in this subarray are serial dilutions of mouse IgG as controls.


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