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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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CAS9 Purified Streptococcus pyogen Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP790148 Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli, 500 pmol (Larger pack size?) ¥998.00 现货
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
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OriGene Data
Species:Streptococcus pyogen Expression Host:E.coli
Expression cDNA Clone or AA Sequence
1
Tag:N-terminal HIS Predicted MW:165.0 kDa
Purity:> 95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:20 uM
Buffer:10mM Tris,300mM NaCl,0.1mM EDTA,1mM DTT, pH7.4, 50% glycerol.
Endotoxin: < 0.5 EU per 1 ?g of the protein by the LAL
BioActivity: In vitro digestion of a DNA fragment with the recombinant S.pyrogene Cas9 nuclease (Cat# TP790148). The input DNA is XmnI-linearized vector (PS100074) was cleaved by with a synthetic sgRNA.
Protein Families:
Protein Pathways:
Protein Data
  Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: RefSeq Size: RefSeq ORF:
LocusID: Cytogenetic:  
Synonyms :
Summary:
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

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