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Home CRISPR/Cas9 AAVS1 Transgene Insertion

AAVS1 Transgene Insertion

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Genome-editing
Knockout Kit
CRISPR/Cas9
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AAVS1
Transgene Insertion
CRISPR/Cas
Starter Kit

Targeted Transgene Insertion into the AAVS1 Locus

Adeno-associated virus integration site 1 (AAVS1) in human genome is a safe harbor for transgene integration. It is transcriptionally active and transgene expression from this site is robust and stable. CRISPR technology is ideal for targeted transgene insertion at the AAVS1 locus.

AAVS1 Safe Harbor knockin system via CRISPR

AAVS1_simple_diagram

This system needs two components:

  1. pCas-Guide-AAVS1, All-in-one CRISPR vector for AAVS1 targeting
  2. pAAVS1-Puro-DNR, donor vector for transgene integration, containing the AAVS1 homologous arms (Gene of interest needs to be cloned)


AAVS1 gRNA Vector

Cat # Product Name Description Price Availability Manual
GE100023 pCas-Guide-AAVS1 AAVS1 gRNA vector, validated AAVS1 targeting sequence cloned in pCas-Guide vector ¥3420 2-3周 Manual

AAVS1 Donor Vector

Cat # Product Name Description Price Availability Manual
GE100024 pAAVS1-Puro-DNR Donor vector with AAVS1 homologous arms cloned, ready for transgene cloning ¥5510 2-3周 Manual
GE100035 pAAVS1-BSD-DNR donor vector with AAVS1 homologous arms cloned (Blasticidin selection), ready for exogenous gene cloning (10 ug) ¥5510 2-3周 Manual

Transgene Knockin Vector Kits

Cat # Product Name Description Price Availability Manual
GE100027 AAVS1 Transgene knockin vector kit Vector kit for targeted transgene insertion into AAVS1 locus, including GE100023, GE100024 and GE100003 ¥8360 2-3周 Manual
GE100036 AAVS1 transgene knockin kit (BSD) Vector kit for targeted transgene insertion into AAVS1 locus (blasticidin selection), including GE100023, GE100035 and GE100003 ¥8360 2-3周 Manual

Scheme of AAVS1 transgene knockin via CRISPR

AAVS1_simple_diagram

Related Products

Cas9 insertion at AAVS1 locus RFP AAVS1 donors
(AAVS1 positive control)
AAVS1-int5 PCR Primer Pairs


 

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